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EGF Recombinant Human Protein, hIgG1-Fc Tag

Catalog number: 10605H01H5

 Related applications: Protein Assays and Analysis

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Description

Epidermal growth factor (EGF), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature soluble form of human EGF (NP_001954.2) (Asn 971-Arg 1023) fused to the Fc region of human IgG1 at the N-terminus.

Activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The EC50 for this effect is typically 20-80 pg/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80..

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: HOMG4, URG, EGF
Gene Symbol: EGF
Molecular Weight: 33 kDa
Product Size: 5 µg
Protein Family: Growth Factors & Receptors
Protein Form: Full Length, Recombinant
Protein Subtype: EGF (Epidermal Growth Factor)
Protein Tag or Fusion: IgG1 Fc Tag
Purity: ≥ 85% by SDS-PAGE
Research Category: Cell Cycle & Proliferation, Other Research
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols

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