Exosome-Human CD9 Isolation Reagent (from cell culture) enables isolation of CD9-positive extracellular vesicles (ECVs) such as exosomes from enriched cell culture media. Following isolation, the specific sub-population of exosomes/ECVs can be further studied using methods like Western blotting, qPCR, or next generation sequencing.
• Minimal hands-on time for specific exosome isolation
• Obtain a highly pure exosome subset
• Protocol is easily scalable
• Compatible with most downstream analysis
Recent studies suggest that the exosome/ECV population consists of several subsets, possibly with different functions. Using the immuno-affinity based Dynabeads™ magnetic separation technology, these subsets can be selectively isolated and studied. The cell culture media must first be enriched for exosomes. This can be done quickly and efficiently using Total Exosome Isolation Reagent (from cell culture media)
or traditional ultracentrifugation. Following enrichment, specific exosomes are isolated by binding to the surface of the magnetic beads.
Dynabeads™ magnetic beads are known for their sensitivity, reproducibility, and stability. Dynabeads™ magnetic separation technology also allows you to 'see' your sample during handling due to the color of the magnetic beads. When the sample tube is placed in the magnet, the bead-bound exosomes are pulled to the side of the tube, allowing for easy separation and buffer changes. In addition, the sample and bead volume can be easily scaled according to your sample size or downstream applications.
Note: It is important to provide sufficient mixing to ensure that the magnetic beads do not settle in the sample tube. We recommend using a mixer which provides both tilting and rotation for optimal mixing.