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HTRA2 Recombinant Human Protein, His Tag

Catalog number: 10619H08E25

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HTRA Serine Peptidase 2 (HTRA2), recombinant human protein is supplied in frozen format. It is suitable for use in protein studies such as enzymetic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of human HTRA2 (O43464-1) (Ala 134-Glu 458) fused to a polyhistide tag at the C-terminus.

Activity: Protease activity demonstrated by HTRA2 cleavage of bovine beta-casein (Sigma, Catalog # C-6905). Incubation of beta-casein at 0.2 µg/ml with Recombinant Human HTRA2 at 0.02 µg/ml (ratio of 10:1) for 60 minutes at 45°C in 50 mM Tris, pH 8.0, which results in >95% cleavage of beta-casein, as revealed by SDS-PAGE.

Formulation: 50 mM Tris, 0.3M NaCl, 1 mM DTT, 0.2 µg/µl BSA, 20% glycerol, pH 7.8.

Reconstitution: No reconstitution needed. Store at -80°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.


Form: Frozen
Purity: ≥ 85% by SDS-PAGE
Species: Human
Expression: E. coli
Gene Symbol: HTRA2
Gene Aliases: OMI, PARK13, PRSS25
Product Size: 5 x 5 µg
Protein Subtype: Proteases
Molecular Weight: 36.5 kDa
Research Category: Other Research
Protein Tag or Fusion: His Tag
Validated Application: Assay Standard

Contents & storage

Store at -80 °C.


Manuals & protocols