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HTRA2 Recombinant Human Protein, His Tag

Catalog number: 10619H08E25

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Description

HTRA Serine Peptidase 2 (HTRA2), recombinant human protein is supplied in frozen format. It is suitable for use in protein studies such as enzymetic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of human HTRA2 (O43464-1) (Ala 134-Glu 458) fused to a polyhistide tag at the C-terminus.

Activity: Protease activity demonstrated by HTRA2 cleavage of bovine beta-casein (Sigma, Catalog # C-6905). Incubation of beta-casein at 0.2 µg/ml with Recombinant Human HTRA2 at 0.02 µg/ml (ratio of 10:1) for 60 minutes at 45°C in 50 mM Tris, pH 8.0, which results in >95% cleavage of beta-casein, as revealed by SDS-PAGE.

Formulation: 50 mM Tris, 0.3M NaCl, 1 mM DTT, 0.2 µg/µl BSA, 20% glycerol, pH 7.8.

Reconstitution: No reconstitution needed. Store at -80°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Other Research
Expression: E. coli
Form: Frozen
Gene Aliases: OMI, PARK13, PRSS25
Gene Symbol: HTRA2
Molecular Weight: 36.5 kDa
Product Line: Sino Biological®
Product Size: 5 x 5 µg
Protein Name: HtrA serine peptidase 2
Protein Subtype: Proteases
Protein Tag or Fusion: His Tag
Purity: ≥ 85% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Store at -80 °C.

Documents

Manuals & protocols