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Hygromycin B (50 mg/mL)

Catalog number: 10687010

Gibco™  Related applications: Insect Cell Culture | Mammalian Cell Culture | Transfection

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Hygromycin B is a water-soluble antibiotic purified from the bacterium Streptomyces hydroscopicus. Hygromycin B acts by binding to the 80S subunit of the bacterial ribosome and inhibiting protein synthesis. Gibco® Hygromycin B is used as a bacterial selection antibiotic in the concentration range of 200–500 µg/mL. This product is supplied as a 50 mg/mL solution.

Resistance to Hygromycin B is conferred by the E. coli hygromycin resistance gene (hyg or hph). Hygromycin B has a different mode of action than Geneticin®, Blasticidin S, or Zeocin™ selection reagents, making it an excellent choice for dual-selection experiments (see selection antibiotics).

Other Choices and More Information
We offer a wide range of antibiotics and antimycotics in both powder and liquid formats.

See the complete list, or find products for:
Contamination control
Eukaryotic and bacterial selection

See recommendations for working concentrations for selection antibiotics.

Learn more about the use of antibiotics and antimycotics in cell culture, and review guidelines for decontaminating cultures.
For Research Use Only. Not for use in diagnostic procedures.


Validated Application: Eukaryotic Selection⁄Stable Cell Line Generation
Agent: Hygromycin
Form: Liquid
Reagent Type: Antibiotic (Selective)
Product Size: 20 mL
Concentration: 50 mg⁄mL
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2 to 8°C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture


Manuals & protocols

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