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MET (c-MET) Recombinant Human Protein (without Catalytic Activity Domain), His Tag

Catalog number: 10692H08H250

 Related applications: Protein Assays and Analysis

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Description

Met proto-oncogene/hepatocyte growth factor receptor (MET/c-MET), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies..

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 932) of human MET (NP_000236) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human HGF at 10 µg/ml (100 µl/well) can bind biotinylated MET. The ED50 of biotinylated Met is 5.28 µg/ml.

Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by SINO Biological.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Growth Factors & Receptors
Research Category: Oncology, Signal Transduction, Stem Cell Research
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: MET, AUTS9, HGFR, RCCP2, c-Met
Gene ID: 4233
Gene Symbol: MET
Kinase Family: MET (HGFR) Kinase Family
Molecular Weight: 103.0 kDa
Product Line: Sino Biological®
Product Size: 5 x 50 µg
Protein Form: Recombinant, Truncated
Protein Name: met proto-oncogene
Protein Subtype: Growth Factor Receptors, Receptor Tyrosine Kinases
Protein Tag or Fusion: His Tag
Purity: ≥ 85% by SDS-PAGE
Species: Human
Validated Application: Assay Standard
Shipping Condition: Room Temperature

Contents & storage

Store at -20 to -70 °C

Documents

Manuals & protocols

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