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CD80 (B7-1) Recombinant Human Protein, His Tag

Catalog number: 10698H08H5

 Related applications: Protein Assays and Analysis

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Description

Cluster of Differentiation 80 (CD80/B7-1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Asn 242) of human CD80 precursor (NP_005182.1) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD80-His at 1 µg/well can bind human CD28-Fc with a linear range of 0.8-4.0 µg/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Drug Development, Immunology, Signal Transduction
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: CD28LG, CD28LG1, LAB7
Gene ID: 941
Gene Symbol: CD80
Molecular Weight: 25.4 kDa
Product Line: Sino Biological®
Product Size: 5 µg
Protein Family: CD Proteins
Protein Form: Cell Surface Protein, Ligand, Recombinant, Truncated
Protein Name: CD80 molecule
Protein Subtype: Other Proteins
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Species: Human
Validated Application: Assay Standard
Shipping Condition: Room Temperature

Contents & storage

Store at -20 to -70 °C

Documents

Manuals & protocols