Defined Keratinocyte-SFM (1X) This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  10744019

 Related applications:

Mammalian Cell Culture

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Defined Keratinocyte-SFM is a serum-free medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers (1,2). The growth-promoting activity of BPE has been replaced by the inclusion of growth promoting agents which enhance consistent product performance. These growth-promoting agents also maintain cell morphology, physiological markers, and growth. The medium is formulated to inhibit fibroblast proliferation and has a low calcium concentration (<0.1 mM) allowing for the isolation and culture of highly enriched populations of undifferentiated keratinocytes.

This formulation contains some human and animal origin components.

A Defined Keratinocyte-SFM Master File has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative. Defined Keratinocyte-SFM consists of a basal liquid medium and frozen supplement which is added at the time of use. The fully supplemented medium has a shelf-life of 10 weeks under normal use conditions. Product performance is confirmed by evaluation of human primary keratinocyte growth.
For Research Use Only. Not for use in diagnostic procedures.


Cell Type: Epithelial Cells, Keratinocytes
Classification: Serum-Free
Form: Liquid
Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Product Size: 500 mL
Serum Level: Serum-Free
Species: Human
Green Features: Sustainable packaging

Contents & storage

• 500 mL Defined Keratinocyte-SFM (store at 2–8°C in the dark)
• 1 mL Defined Keratinocyte-SFM Growth Supplement (store at -5 to -20°C in the dark)

Store the complete medium at 2–8°C in the dark.


Manuals & protocols