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CD70 Recombinant Human Protein, hIgG1-Fc Tag

Catalog number: 10780H01H50

 Related applications: Protein Assays and Analysis

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Description

Cluster of Differentiation 70 (CD70), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Gln 39-Pro 193) of human CD70 (NP_001243.1) fused to the Fc region of human IgG1 at the N-terminus with a polypeptide linker.

Activity: Measured by its binding ability in a functional ELISA.
1. Immobilized mouse CD27 at 2 µg/ml(100 µl/well) can bind human CD70 with a linear range of 78-1250 pg/ml.
2. Immobilized mouse CD27-Fc at 2 µg/ml(100 µl/well) can bind biotinylated human CD70 with a linear range of 0.39-12.5 ng/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Immunology
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: CD70, CD70 / CD27L / TNFSF7, CD70 / CD27L / TNFSF7G, TNFSF7
Gene ID: 970
Gene Symbol: CD70
Molecular Weight: 45.5 kDa
Product Line: Sino Biological®
Product Size: 50 µg
Protein Family: CD Proteins
Protein Form: Cell Surface Protein, Ligand, Recombinant, Truncated
Protein Name: CD70 molecule
Protein Subtype: TNF (Tumor Necrosis Factor)
Protein Tag or Fusion: IgG1 Fc Tag
Purity: ≥ 90% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Store at -20 to -70 °C

Documents

Manuals & protocols