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EFNB2 (EphrinB2) Recombinant Human Protein, hIgG1-Fc.His Tag

Catalog number: 10881H03H50

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Description

Ephrin-B2 (EFNB2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ala 229) of human EFNB2 (NP_004084.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA . Immobilized human EFNB4-His at 2 µg/ml(100 µl/well) can bind human EFNB2-Fc with a linear range of 1-25 ng/ml. Immobilized human EFNB4 at 2 µg/ml(100 µl/well) can bind human EFNB2-Fc with a linear range of 1-125 ng/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: EFNB2, RP11-272L14.1, EPLG5, HTKL, Htk-L, LERK5, MGC126226, MGC126227, MGC126228
Gene ID: 1948
Gene Symbol: EFNB2
Molecular Weight: 50.3 kDa
Product Line: Sino Biological®
Product Size: 50 µg
Protein Family: Kinases & Inhibitors
Protein Form: Cell Surface Protein, Ligand, Recombinant, Truncated
Protein Name: ephrin-B2
Protein Subtype: Ephrins
Protein Tag or Fusion: His Tag, IgG1 Fc Tag
Purity: ≥ 90% by SDS-PAGE
Research Category: Development, Neurobiology, Oncology, Signal Transduction
Species: Human
Validated Application: Assay Standard

Contents & storage

Store at -20 to -70 °C

Documents

Manuals & protocols

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