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MAP1D Recombinant Human Protein, His Tag

Catalog number: 10883H08B50

 Related applications: Protein Assays and Analysis

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Description

Methionyl aminopeptidase type 1D (mitochondrial) (METAP1D), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the human METAP1D (NP_954697.1) (Arg 44-Ala 335) fused to with a polyhistidine tag at the C-terminus and an initial Met an the N-terminus.

Activity:
Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC. The resulting Gly-Pro-AMC is cleaved by human DPP4/CD26
. The specific activity is >30 pmoles/min/µg
.

Formulation: Lyophilized in 50 mM Tris, 100 mM NaCl, pH 8.0, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Other Proteins
Research Category: Oncology
Endotoxin Level: < 1 EU/µg
Expression: Insect
Form: Lyophilized
Gene Aliases: MAP1D
Gene Symbol: MAP1D
Molecular Weight: 33.4 kDa
Product Line: Sino Biological®
Product Size: 50 µg
Protein Name: methionyl aminopeptidase type 1D (mitochondrial
Protein Subtype: Proteases
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols