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Invitrogen™

SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

Catalog number: 10928042
Have Questions?
Invitrogen™

SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

Catalog number: 10928042
Have Questions?
Catalog Number
10928042
Unit Size
100 rxns
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
10928042100 rxns
-
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SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR. This system combines RT/Platinum Taq mix and 2X reaction mix. The RT/Platinum Taq mix contains a mixture of SuperScript II Reverse Transcriptase and Platinum Taq DNA Polymerase. The 2X reaction mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers.

All components for cDNA synthesis and PCR are combined in a single tube with gene-specific primers and target RNA. Reverse transcription automatically follows PCR cycling without additional steps.

Note: For superior one-step RT-PCR performance, we recommend the SuperScript IV One-Step RT-PCR System or SuperScript IV One-Step RT-PCR System with ezDNase. The SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched product yields, specificity, and sensitivity in less time and for a broad target range, even with suboptimally pure RNA samples.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Gel Electrophoresis
Format
Kit
PCR Method
1-step RT-PCR
Polymerase
Platinum Taq DNA Polymerase
Reaction Speed
30 to 50 min.
Technique
1-step RT-PCR
Optimal Reaction Temperature
55°C to 60°C
Reverse Transcriptase
SuperScript II
Ribonuclease H Activity
Reduced
Shipping Condition
Wet Ice
Final Product Type
PCR Amplified cDNA
Hot Start
Built-In Hot Start
No. of Reactions
100 Reactions
Reaction Format
Master Mix
Reagent Type
Reverse Transcription
Size (Final Product)
Up to 7 kb
Starting Material
RNA

Contents & Storage

• SuperScript II RT/Platinum Taq DNA Polymerase mix (100 μL)
• 2X Reaction mix (3 x 1 mL)
• 5 mM magnesium sulfate (500 μL)
• 50 mM magnesium sulfate (1 mL)

Store at –20°C.

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