For superior RT-PCR performance, upgrade to the new SuperScript IV One-Step RT-PCR System.
Invitrogen™

SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

Catalog number:  10928042

Related applications:

Reverse Transcription

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Description

The SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR. This system combines RT/ Platinum Taq Mix and 2X Reaction Mix. The RT/ Platinum Taq Mix contains a mixture of SuperScript II Reverse Transcriptase and Platinum Taq DNA Polymerase. The 2X Reaction Mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers. All components for cDNA synthesis and PCR are combined in a single tube with gene-specific primers and target RNA. Reverse transcription automatically follows PCR cycling without additional steps.

Note: For superior one-step RT-PCR performance, we recommend the SuperScript IV One-Step RT-PCR System or SuperScript IV One-Step RT-PCR System with ezDNase. The SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched product yields, specificity, and sensitivity in less time and for a broad target range, even with suboptimally pure RNA samples.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Hot Start: Built-In Hot Start
Includes: 100μL SuperScript II RT/Platinum Taq DNA Polymerase mix; 3 x 1mL 2X reaction mix; 500μL 5mM magnesium sulfate; 1mL 50mM magnesium sulfate
Primer Probe Compatibility: Unlabeled Oligos
Quantity: 100 reactions
Reverse Transcriptase: SuperScript™ II
Sample Type: RNA
Shipping Condition: Wet Ice
Concentration: 2 X
Detection Method: Gel Electrophoresis
Fidelity (vs. Taq): 2 X
Format: Tube
GC-Rich PCR Performance: High
No. of Reactions: 100 Reactions
No. of Tests: 100 Reactions
PCR Method: 1-Step RT-PCR
Polymerase: Platinum™ Taq DNA Polymerase
Product Line: Platinum™, SuperScript™
Product Type Specs: One-Step RT-PCR System
Reaction Speed: Fast
Size (Final Product): 3 kb or less
Sufficient For: 100 Reactions
Volume: 1 mL

Contents & storage

SuperScript™ One-Step RT-PCR System components are listed in Table 1. Store at -20°C. Guaranteed stable for 6 months when properly stored.