Informational:For superior RT-PCR performance, upgrade to the new SuperScript IV One-Step RT-PCR System.
Invitrogen™

SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

Catalog number: 10928042
Invitrogen™

SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

Catalog number: 10928042
Catalog Number
10928042
Unit Size
100 rxns
Price (USD)
Price: 842.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
10928042100 rxns
Price: 842.00
Your Price:
-
Product Overview
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The SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR. This system combines RT/ Platinum Taq Mix and 2X Reaction Mix. The RT/ Platinum Taq Mix contains a mixture of SuperScript II Reverse Transcriptase and Platinum Taq DNA Polymerase. The 2X Reaction Mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers. All components for cDNA synthesis and PCR are combined in a single tube with gene-specific primers and target RNA. Reverse transcription automatically follows PCR cycling without additional steps.

Note: For superior one-step RT-PCR performance, we recommend the SuperScript IV One-Step RT-PCR System or SuperScript IV One-Step RT-PCR System with ezDNase. The SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched product yields, specificity, and sensitivity in less time and for a broad target range, even with suboptimally pure RNA samples.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
2 X
Detection Method
Gel Electrophoresis
Fidelity (vs. Taq)
2 X
Format
Kit
GC-Rich PCR Performance
High
Hot Start
Built-In Hot Start
No. of Reactions
100 Reactions
PCR Method
1-Step RT-PCR
Polymerase
Platinum Taq
Reaction Format
Master Mix
Reaction Speed
Standard
Reagent Type
Reverse Transcription
Sample Type
RNA
Size (Final Product)
3 kb or Less
Starting Material
RNA
Technique
1-Step RT-PCR
Volume
1 mL
Includes
100μL SuperScript II RT/Platinum Taq DNA Polymerase mix; 3 x 1mL 2X reaction mix; 500μL 5mM magnesium sulfate; 1mL 50mM magnesium sulfate
No. of Tests
100 Reactions
Primer Probe Compatibility
Unlabeled Oligos
Product Line
Platinum™, SuperScript™
Product Type
One-Step RT-PCR System
Quantity
100 Reactions
Reverse Transcriptase
SuperScript™ II
Shipping Condition
Wet Ice
Sufficient For
100 Reactions

Contents & Storage

SuperScript™ One-Step RT-PCR System components are listed in Table 1. Store at -20°C. Guaranteed stable for 6 months when properly stored.