SuperScript® One-Step RT-PCR System with Platinum® Taq DNA Polymerase

Catalog number: 10928042

Invitrogen™  Related applications: Reverse Transcription

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Description

The SuperScript® One-Step RT-PCR System with Platinum® Taq DNA Polymerase is designed for the convenient, sensitive, and reproducible detection and analysis of RNA molecules by RT-PCR. Components for both cDNA synthesis and PCR are combined in a single tube, using gene-specific primers and target RNAs from either total RNA or mRNA. Reverse transcription automatically follows PCR cycling without additional steps.

Using the SuperScript® One-Step System
The system consists of two major components: RT/ Platinum® Taq Mix and 2X Reaction Mix. The RT/ Platinum® Taq Mix contains a mixture of SuperScript® II Reverse Transcriptase and Platinum® Taq DNA Polymerase for optimal cDNA synthesis and PCR amplification. SuperScript® II RT is a modified version of Moloney Murine Leukemia Virus (M-MLV) RT, engineered to reduce RNase H activity and increase thermal stability. Platinum® Taq DNA polymerase is Taq DNA polymerase complexed with a proprietary antibody that inhibits polymerase activity at ambient temperatures. The antibody is denatured and polymerase activity is restored during the denaturation step in PCR cycling at 94°C. This provides an automatic "hot start" in PCR, increasing sensitivity, specificity, and yield. The 2X Reaction Mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers. This convenient 2X format allows further addition of template and primer at any desired concentration.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

PCR Method: 1-Step RT-PCR
Reverse Transcriptase: SuperScript® II
Polymerase: Platinum® Taq DNA Polymerase
Hot Start: Built-In Hot Start
Sample Type (General): RNA
Detection Method: Gel Electrophoresis
Final Product Size(s): 3 kb or less
Primer-Probe Compatibility: Unlabeled Oligos
Product Size: 100 reactions
Shipping Condition: Wet Ice

Contents & storage

SuperScript® One-Step RT-PCR System components are listed in Table 1. Store at -20°C. Guaranteed stable for 6 months when properly stored.

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