TIMP1 Recombinant Human Protein

Description

TIMP metallopeptidase inhibitor 1 (TIMP1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the human TIMP1 (NP_003245.1) (Met 1-Ala 207).

Activity: Measured by its ability to inhibit human MMP2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH₂. The IC₅₀ value is approximately 6 nM.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

Specifications

Endotoxin Level:	< 1 EU/µg
Expression:	Mammalian
Form:	Lyophilized
Gene Aliases:	TIMP1M, RP1-230G1.3, CLGI, EPA, EPO, FLJ90373, HCI, TIMP
Gene Symbol:	TIMP1
Molecular Weight:	22 kDa
Product Line:	Sino Biological®
Product Size:	5 x 5 µg
Protein Family:	Proteases Peptidases & Inhibitors
Protein Name:	TIMP meallopeptidase inhibitor 1
Protein Subtype:	TIMP
Protein Tag or Fusion:	Untagged
Purity:	≥ 90% by SDS-PAGE
Research Category:	Angiogenesis, Inflammation, Neurobiology, Oncology
Species:	Human

Contents & storage