DMEM, high glucose, NEAA, no glutamine This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 10938025

Gibco™  Related applications: Mammalian Cell Culture

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Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

• High Glucose• L-glutamine
• Non Essential Amino Acids• Sodium Pyruvate
• Phenol Red• HEPES

The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.


Glutamine: No Glutamine
Phenol Red Indicator: Phenol Red
Form: Liquid
Glucose: High Glucose
Serum Supplementation: Standard Serum Supplementation
Sodium Pyruvate Additive: No Sodium Pyruvate
pH Range: 7.0 - 7.2
Osmolality: 310 - 360 mOsm/kg
Concentrated: 1 X
Product Size: 500 mL
Classification: Animal Origin-Free
Volume (Metric): 500 ml
Culture Environment: CO2
Supplementation Required: Serum
Sodium Bicarbonate Buffer: Sodium Bicarbonate
Green Features: Fewer resources used, Less waste
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.


Manuals & protocols

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