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Invitrogen™

Platinum™ Taq DNA Polymerase, Brasil

Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.
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카탈로그 번호반응 수
10966020100 Reactions
10966030500 Reactions
109661745000 Reactions
1096618225,000 Reactions
카탈로그 번호 10966020
제품 가격(KRW)
-
반응 수:
100 Reactions
Platinum Taq DNA Polymerase is a convenient and reliable hot start thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The hot start property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step.

Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template–dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity (but not 3' to 5' exonuclease activity). PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications.

Features

  • Assembly of PCR reactions at room temperature
  • Hot-start kinetics reduce nonspecific primer annealing and improve product yield
  • May be used for PCR products up to 10 kb
  • Optional KB Extender provides more versatility in PCR assays

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • RT-PCR
For Research Use Only. Not for use in diagnostic procedures.
사양
Fidelity (Taq 대비)1X
핫 스타트Built-In Hot Start
반응 수100 Reactions
오버행3'-A
중합효소Platinum Taq DNA Polymerase
수량100 reactions
배송 조건Dry Ice
크기(최종 제품)10 kb or less
시작 물질DNA
농도5 U/μL
용도(애플리케이션)Hot-start PCR
GC-Rich PCR PerformanceMedium
반응 속도Standard
Unit SizeEach
구성 및 보관
• Platinum Taq DNA Polymerase, 20 μL
• 10X PCR Buffer (without magnesium), 1.25 mL
• 50 mM MgCl2, 1 mL

Store at -15°C to -25°C in a non-frost-free freezer.