Platinum™ Taq DNA Polymerase, Brasil
Product Image
Citations & References (2)
Invitrogen™

Platinum™ Taq DNA Polymerase, Brasil

Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.
Have Questions?
Change viewbuttonViewtableView
Catalog NumberNo. of Reactions
10966030500 Reactions
10966020100 Reactions
109661745000 Reactions
1096618225,000 Reactions
Catalog number 10966030
Price (MXN)
3,366.31
Each
Add to cart
No. of Reactions:
500 Reactions
Price (MXN)
3,366.31
Each
Add to cart
Platinum Taq DNA Polymerase is a convenient and reliable hot start thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The hot start property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step.

Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template–dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity (but not 3' to 5' exonuclease activity). PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications.

Features

  • Assembly of PCR reactions at room temperature
  • Hot-start kinetics reduce nonspecific primer annealing and improve product yield
  • May be used for PCR products up to 10 kb
  • Optional KB Extender provides more versatility in PCR assays

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • RT-PCR
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Fidelity (vs. Taq)1X
Hot StartBuilt-In Hot Start
No. of Reactions500 Reactions
Overhang3'-A
PolymerasePlatinum Taq DNA Polymerase
Quantity500 reactions
Shipping ConditionDry Ice
Size (Final Product)10 kb or less
Starting MaterialDNA
Concentration5 U/μL
For Use With (Application)Hot-start PCR
GC-Rich PCR PerformanceMedium
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• Platinum Taq DNA Polymerase, 100 μL
• 10X PCR Buffer (without magnesium), 2 x 1.25 mL
• 50 mM MgCl2, 1 mL

Store at -15°C to -25°C in a non-frost-free freezer.

Frequently asked questions (FAQs)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

View all Platinum™ Taq DNA Polymerase, Brasil FAQs

Citations & References (2)

Citations & References
Abstract
Dynamin 2 mutations associated with human diseases impair clathrin-mediated receptor endocytosis.
Authors:Bitoun M, Durieux AC, Prudhon B, Bevilacqua JA, Herledan A, Sakanyan V, Urtizberea A, Cartier L, Romero NB, Guicheney P,
Journal:Hum Mutat
PubMed ID:19623537
Dynamin 2 (DNM2) is a large GTPase involved in the release of nascent vesicles during endocytosis and intracellular membrane trafficking. Distinct DNM2 mutations, affecting the middle domain (MD) and the Pleckstrin homology domain (PH), have been identified in autosomal dominant centronuclear myopathy (CNM) and in the intermediate and axonal forms ... More
Utility and accuracy of template-directed dye-terminator incorporation with fluorescence-polarization detection for genotyping single nucleotide polymorphisms.
Authors: Akula N; Chen Y S; Hennessy K; Schulze T G; Singh G; McMahon F J;
Journal:Biotechniques
PubMed ID:12019780
There are little independent data available about how well single nucleotide polymorphism (SNP) genotyping technologies perform in the typical molecular genetics laboratory. We evaluated the utility and accuracy of a widely used technology, template-directed dye-terminator incorporation with fluorescence-polarization detection (FP-TDI), in a sample of 177 SNPs selected solely on the ... More