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CD9 Recombinant Human Protein, His Tag

Catalog number: 11029H08H250

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Description

CD9 Molecule (CD9), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the second extracellular domain (Ser 112-Ile 195) of human CD9 (NP_001760.1) fused to a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

Activity: Measured by its binding ability in a functional ELISA.

1. Immobilized human CD19 at 2 µg/ml (100 µl/well) can bind biotinylated human CD9 with a linear ranger of 0.032-0.8 µg/ml.

2. Immobilized mouse CD19 at 20 µg/ml (100 µl/well) can bind biotinylated human CD9 with a linear ranger of 0.032-0.8 µg/ml.

3.Immobilized human CD21 at 2 µg/ml (100 µl/well) can bind biotinylated human CD9. The EC50 of biotinylated human CD9 is 1.76 µg/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: CD Proteins
Research Category: Cell Cycle & Proliferation, Immunology, Inflammation, Oncology, Virology
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: MIC3, TSPAN29, GIG2, CD9
Gene Symbol: CD9
Molecular Weight: 11 kDa
Product Line: Sino Biological®
Product Size: 5 x 50 µg
Protein Name: CD9 Molecule
Protein Subtype: Other Proteins
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols