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NGF Recombinant Human Protein

Catalog number: 11050HNAC5

 Related applications: Protein Assays and Analysis

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Manufactured by Sino Biological (China); Distributed by Life Technologies.

Nerve growth factor (NGF) recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of human NGF (NP_002497.2) (Ser 122-Arg 239).

N-terminal Sequence Analysis: Ser 122

Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is typically 0.4-1.0 ng/mL.

Formulation: Lyophilized in 50 mM NaAc, 150 mM NaCl, pH 5.5, 5% mannitol, 5% trehalose, 0.02% Tween®-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

As the first one of a series of neurotrophic factors, nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF, was demonstrated to exhibits the growth stimulating activity of NGF. β-NGF is a target-derived secreted homodimeric protein of two 120-amino acid mature polypeptides after proteolytic removal of the signal and pro peptide. NGF acts via at least two receptors on the surface of cells, TrkA and p75 receptors to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In additon to its neurotropic activities, studies indicated that NGF may also have an important role in the regulation of the immune system. The synthesis of β-NGF in astrocytes is enhanced by various other cytokines including IL-1, TNF-α, PDGF and TGF-β .Defects in β-NGF are the cause of hereditary sensory and autonomic neuropathy type 5 (HSAN5) characterized by degeneration of dorsal root and autonomic ganglion cells, as well as sensory and/or autonomic abnormalities.

You may also be interested in the following NGF products:
Enzyme & Protein Assays
Primary Antibodies
Gene Expression Assays
For Research Use Only. Not for use in diagnostic procedures.


Form: Lyophilized
Purity: ≥ 95% by SDS-PAGE
Species: Human
Expression: Mammalian
Gene Symbol: NGF
Gene Aliases: NGF, Beta-NGF, HSAN5, MGC161426, MGC161428, NGFB
Product Size: 5 µg
Protein Form: Full Length, Recombinant
Protein Family: Growth Factors & Receptors
Endotoxin Level: < 1 EU/µg
Protein Subtype: NGFs (Nerve Growth Factors)
Molecular Weight: 13.2 kDa
Research Category: Immunology, Development, Neurobiology
Protein Tag or Fusion: Untagged
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.


Manuals & protocols

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