Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum
supplementation by at least 50% with no change in cell growth rate or morphology. Opti-MEM™ I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM™ I Reduced-Serum Medium modifications
for a range of cell culture applications.
This Opti-MEM™ is modified as follows:
The complete formulation is confidential. For more information, please contact Technical Services
.Using Opti-MEM™ Reduced-Serum Medium
Opti-MEM™ I medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2
environment to maintain physiological pH.cGMP manufacturing and quality system
For supply chain continuity, we manufacture Opti-MEM™ Reduced-Serum Medium at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.