MEM
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MEM
Gibco™

MEM

MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be usedRead more
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Catalog NumberQuantity
11095080500 mL
1109509810 x 500 mL
110950721000 mL
110951146 x 1000 mL
Catalog number 11095080
Price (USD)/ Each
34.65
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37.45
Save 2.80 (7%)
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Quantity:
500 mL
Customize this product
Price (USD)/ Each
34.65
Online Exclusive
37.45
Save 2.80 (7%)
Add to cart

MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This MEM is modified as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red 


The complete formulation is available.

Using MEM
MEM was developed by Harry Eagle, based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell TypePrimary Rat Astrocytes
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeMEM (Minimum Essential Medium)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesLow Glucose, Glutamine, Phenol Red
Without AdditivesNo HEPES, No Sodium Pyruvate
Unit SizeEach
Contents & Storage
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture