Invitrogen™

Dynabeads™ CD8

Catalog number: 11147D
Invitrogen™

Dynabeads™ CD8

Catalog number: 11147D
Catalog Number
11147D
Unit Size
5 mL
Price (USD)
Price: 1,274.00
Online price: 1,006.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11147D5 mL
Price: 1,274.00
Online price: 1,006.65
Your price:
-
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Dynabeads™ CD8 are superparamagnetic beads coupled with an anti-human CD8 antibody that enable easy isolation or depletion of human CD8+ T cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads™ CD8:

• Fast and efficient isolation of CD8+ T cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD8+ T cells for molecular downstream assays
• Fast and efficient depletion of CD8+ T cells from any sample

About Dynabeads™ CD8
Dynabeads™ CD8 are uniform, superparamagnetic beads (4.5 μm diameter) coated with a primary monoclonal antibody specific for the CD8 membrane antigen mainly expressed on human cytotoxic T cells. Because of the bead size, Dynabeads™ CD8 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads™ CD8 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD8+ cells for cell-based applications, or you need to check your samples with flow cytometry, check out our total human CD8 product offerings.

Magnetic bead–based separation offers easy handling
Dynabeads™ CD8 is added to the sample under continuous mixing to optimize the binding of the Dynabeads™ to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

Learn more about Dynabeads™ products
• Find Dynabeads™ products for a whole range of applications
• Find magnets for Dynabeads™ separations.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
T Cells (CD8)
Product Type
Antibody Coated Bead
Isolation Technology
Depletion, positive isolation for molecular applications
No. of Cells
Processes ∼2 x 109 cells total
Output Viability
>95%
Product Line
DYNAL™, Dynabeads™
Purity or Quality Grade
Research Grade
Sample Type
PBMC, Blood
Shipping Condition
Room Temperature
Starting Material Cell No.
1 x 107 PBMCs per isolation
Target Species
Human
Diameter (Metric)
4.5 μm

Contents & Storage

This Product Contains: 5 mL Dynabeads™ CD8 coated with anti-CD8 monoclonal antibody.

Storage Conditions: 2°C to 8°C. Do not freeze.

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