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Medium 199 This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  11150067

Related applications:

Mammalian Cell Culture

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Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium and rat lens tissues. We offer a variety of Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This M199 is modified as follows:
WithWithout
• Phenol Red• HEPES
• L-glutamine

The complete formulation is available.

Using Medium 199
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product contains Earle's salts. Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
Medium 199 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Medium 199 product made in our Scotland facility (31150-030). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
For In Vitro Diagnostic Use.

Specifications

Form: Liquid
Glucose: Low Glucose
Glutamine: L-Glutamine
Green Features: Sustainable packaging
HEPES Buffer: No HEPES
Phenol Red Indicator: Phenol Red
Product Line: Gibco®
Product Size: 10 x 500 mL
Shipping Condition: Room Temperature
Sodium Pyruvate Additive: No Sodium Pyruvate
Volume (Metric): 500 ml

Contents & storage

Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Media formulations