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gp120 (fragment of env) Recombinant HIV Protein, His Tag, Active

Catalog number: 11233V08H250

 Related applications: Protein Assays and Analysis

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HIV-1 glycoprotein 120 (gp120, fragment of env), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the HIV-1 surface glycoprotein gp120 (Ala 29-Glu 503), which is cleaved from the full length envelope glycoprotein gp160, fused to a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD4-His at 2 µg/well can bind biotinylated HIV-1 GP120 with a linear range of 0.078-1.25 µg/ml.

Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 25 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.


Form: Lyophilized
Purity: ≥ 95% by SDS-PAGE
Gene ID: 155971
Species: HIV
Expression: Mammalian
Gene Symbol: gp120
Gene Aliases: gp120, SU, Surface glycoprotein 120
Product Size: 5 x 50 µg
Protein Form: Recombinant
Endotoxin Level: < 1 EU/µg
Protein Subtype: Viral Proteins
Molecular Weight: 54.6 kDa
Research Category: Immunology, Virology
Protein Tag or Fusion: His Tag
Validated Application: Assay Standard

Contents & storage

Store at -20 to -70 °C


Manuals & protocols