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Invitrogen™

Platinum™ Taq DNA Polymerase High Fidelity

Catalog number:  11304029

Related applications:

PCR

| Chromatin Biology

| Methylation Analysis

| Sanger Sequencing

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Description

Platinum® Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´→5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum® automatic 'hot-start' technology. Features of Platinum® Taq DNA Polymerase, High Fidelity:

Fidelity—greater than six times higher fidelity than Taq DNA polymerase
Amplicon size—amplification of fragments up to 15 kb (see figure)
Convenience—room temperature reaction assembly
Applications—amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR

Unit definition
One unit of Platinum® Taq DNA Polymerase, High Fidelity, is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Exonuclease Activity: 3' - 5', 5' - 3'
Hot Start: Built-In Hot Start
Product Line: Platinum®
Quantity: 500 reactions
Shipping Condition: Dry Ice
Fidelity (vs. Taq): 6 X
GC-Rich PCR Performance: Low
Overhang: Mixed
Polymerase: Platinum® Taq DNA Polymerase High Fidelity
Reaction Format: Separate Components
Reaction Speed: Standard
Size (Final Product): 20 kb or less

Contents & storage

• 1 x 100 µL Platinum Taq DNA Polymerase High Fidelity (5 U/µL)
• 1 x 2.25 mL 10X High Fidelity Buffer [600 mM Tris-SO4 (pH 8.9), 180 mM (NH4)2SO4]
• 1 x 1 mL 50 mM MgSO4

Store at -10°C to -30°C.