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ADN polymérase <i>Taq</i> Platinum&trade;, haute fidélité
Citations et références (5)
Invitrogen™

ADN polymérase Taq Platinum™, haute fidélité

L’ADN polymérase Platinum™ Taq haute fidélité est idéale pour l’amplification de fragments d’ADN quand des rendements élevés et une amplificationAfficher plus
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RéférenceNbre de réactions
113041025000 réactions
11304011100 réactions
11304029500 réactions
Référence 11304102
Prix (EUR)
7 398,65
Offre exceptionnelle en ligne
10 570,00
Économisez 3 171,35 (30%)
Each
Nbre de réactions:
5000 réactions
Grand volume ou format personnalisé
Prix (EUR)
7 398,65
Offre exceptionnelle en ligne
10 570,00
Économisez 3 171,35 (30%)
Each
L’ADN polymérase Platinum™ Taq haute fidélité est idéale pour l’amplification de fragments d’ADN quand des rendements élevés et une amplification robuste sont requis. La haute fidélité est fournie par un mélange d’ADN polymérase Platinum™ Taq et de polymérase à activité corrective (activité d’exonucléase 3´ → 5´) de l’espèce Pyrococcus GB-D. La spécificité de la PCR est améliorée par l’incorporation de la technologie Platinum™ à “démarrage à chaud” automatique. Caractéristique de l’ADN polymérase Platinum™ Taq, haute fidélité :

• Fidélité : une fidélité plus de six fois supérieure à celle de l’ADN polymérase Taq
• Taille des amplicons : amplification de fragments jusqu’à 15 kb (voir l’illustration)
• Confort : assemblée de réaction à température ambiante
• Applications : amplification d’ADN provenant de modèles génomiques, viraux et plasmides complexes ; et RT-PCR.

Définition d’une unité
Une unité de l’ADN polymérase Platinum™ Taq haute fidélité représente la quantité d’enzyme requise pour incorporer 10 nmol de désoxyribonucléotide dans l’ADN en 30 minutes à 74°C.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Fidélité (par rapport à Taq)6 X
Hot StartFonctionnalité Hot-Start (démarrage à chaud) intégrée
Nbre de réactions5000 réactions
En surplombMixte, 3’-A
PolyméraseADN polymérase haute fidélité Platinum Taq
Type de produitADN polymérase
Quantité5,000 reactions
Format de réactionComposants séparés
Conditions d’expéditionGlace carbonique
Taille (produit final)20 kb ou moins
Méthode de détectionAmorce-sonde
À utiliser avec (application)Clonage, High-fidelity PCR
GC-Rich PCR PerformanceFaible
Méthode PCRRT-qPCR en 1 étapes
Vitesse de réactionÉtalon
Unit SizeEach
Contenu et stockage
• 1 x 1 000 µl d’ADN polymérase haute fidélité Platinum Taq (5 U/µl)
• 1 x 50 ml de tampon haute fidélité 10X [600 mM de Tris-SO4 (pH 8,9), 180 mM (NH4)2SO4]
• 1 x 25 ml de MgSO4 à 50 mM

Conserver entre -10°C et -30°C.

Foire aux questions (FAQ)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

View all ADN polymérase Taq Platinum™, haute fidélité FAQs

Citations et références (5)

Citations et références
Abstract
ND5 is a hot-spot for multiple atypical mitochondrial DNA deletions in mitochondrial neurogastrointestinal encephalomyopathy.
Authors:Nishigaki Y, Marti R, Hirano M,
Journal:Hum Mol Genet
PubMed ID:14613972
'Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) is an autosomal recessive multisystem disorder associated with depletion, multiple deletions and site-specific point mutations of mitochondrial DNA (mtDNA). MNGIE is caused by loss-of-function mutations in the gene encoding thymidine phosphorylase (TP; endothelial cell growth factor 1). Deficiency of TP leads to dramatically elevated levels of ... More
Relation between kidney function, proteinuria, and adverse outcomes.
Authors:Hemmelgarn BR, Manns BJ, Lloyd A, James MT, Klarenbach S, Quinn RR, Wiebe N, Tonelli M
Journal:JAMA
PubMed ID:20124537
'The current staging system for chronic kidney disease is based primarily on estimated glomerular filtration rate (eGFR) with lower eGFR associated with higher risk of adverse outcomes. Although proteinuria is also associated with adverse outcomes, it is not used to refine risk estimates of adverse events in this current system.' ... More
Factor B and the mitochondrial ATP synthase complex.
Authors: Belogrudov Grigory I; Hatefi Youssef;
Journal:J Biol Chem
PubMed ID:11744738
Factor B is a subunit of the mammalian ATP synthase complex, whose existence has been controversial. This paper describes the molecular and functional properties of a recombinant human factor B, which when added to bovine submitochondrial particles depleted of their factor B restores the energy coupling activity of the ATP ... More
Human protein factory for converting the transcriptome into an in vitro-expressed proteome,.
Authors:Goshima N, Kawamura Y, Fukumoto A, Miura A, Honma R, Satoh R, Wakamatsu A, Yamamoto J, Kimura K, Nishikawa T, Andoh T, Iida Y, Ishikawa K, Ito E, Kagawa N, Kaminaga C, Kanehori K, Kawakami B, Kenmochi K, Kimura R, Kobayashi M, Kuroita T, Kuwayama H, Maruyama Y, Matsuo K, Minami K, Mitsubori M, Mori M, Morishita R, Murase A, Nishikawa A, Nishikawa S, Okamoto T, Sakagami N, Sakamoto Y, Sasaki Y, Seki T, Sono S, Sugiyama A, Sumiya T, Takayama T, Takayama Y, Takeda H, Togashi T, Yahata K, Yamada H,
Journal:Nat Methods
PubMed ID:19054851
Appropriate resources and expression technology necessary for human proteomics on a whole-proteome scale are being developed. We prepared a foundation for simple and efficient production of human proteins using the versatile Gateway vector system. We generated 33,275 human Gateway entry clones for protein synthesis, developed mRNA expression protocols for them ... More
Mutation screening in 86 known X-linked mental retardation genes by droplet-based multiplex PCR and massive parallel sequencing.
Authors:Hu H, Wrogemann K, Kalscheuer V, Tzschach A, Richard H, Haas SA, Menzel C, Bienek M, Froyen G, Raynaud M, Van Bokhoven H, Chelly J, Ropers H, Chen W
Journal:Hugo J
PubMed ID:21836662
Massive parallel sequencing has revolutionized the search for pathogenic variants in the human genome, but for routine diagnosis, re-sequencing of the complete human genome in a large cohort of patients is still far too expensive. Recently, novel genome partitioning methods have been developed that allow to target re-sequencing to specific ... More