This product will be discontinued by May 1, 2016. We recommend you switch to Platinum® PCR SuperMix.

Platinum® PCR SuperMix 96, non-skirted

Catalog number: 11306073

Invitrogen™  Related applications: PCR

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Description

Platinum® PCR SuperMix 96 provides qualified reagents for the amplification of nucleic acid templates by polymerase chain reaction (PCR). The reagents are prealiquoted into 96-well microtiter plates (nonskirted) for high-throughput applications as well as general ease of use.

Each plate well contains anti-Taq DNA polymerase antibody, Mg++, deoxyribonucleotide triphosphates, and recombinant Taq DNA Polymerase. Due to specific binding of the anti-Taq antibody, polymerase activity is inhibited at room temperature. Activity is restored after a denaturation step in PCR cycling at 94°C, providing an automatic hot start in PCR and thereby
improving PCR specificity and yield.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: Platinum® Taq DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: SuperMix or Master Mix
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Product Size: 480 reactions
Exonuclease Activity: 5' - 3'
Shipping Condition: Dry Ice

Contents & storage

Each 22.5 µl volume of Platinum® PCR SuperMix 96 is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solution. Reagents are provided for 5 × 96 amplification reactions of 25 µl each.

480 rxns: 5 nonskirted plates containing 22.5 µL of Platinum® PCR SuperMix

Sore at -20 °C in a non-frost-free freezer.

Documents

Manuals & protocols