ElectroMAX™ DH5α-E™ Competent Cells

Catalog number: 11319019

Invitrogen™  Related applications: Transformation

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Description

ElectroMAX™ DH5α-E™ Competent Cells are derived from the DH5α™ strain and are suitable for transformation by electroporation. They may be used in procedures requiring high transformation efficiencies, such as generation of cDNA libraries or in transformations with limited input DNA. ElectroMAX™ DH5α-E™ cells offer:

• >1 x 1010 transformants/µg efficiency with electroporation
• Greatly increased plasmid yield and quality due to endA1 mutation
• High-efficiency transformation with plasmids 30 kb in size
• Blue/white screening of recombinant clones due to lacZΔM15
• Ensured insert stability due to recA1 mutation
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Electrocompetent
Species: E. coli
Format: Tube(s)
Transformation Efficiency: > 1x10^10
Bacterial or Yeast Strain: DH5α™
Plasmid Type: May be used for plasmids > 20 kb
Product Size: 0.5 mL
Blue-White Screening: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Reduces Recombination: Yes
Cloning Methylated DNA: Yes
Improves Plasmid Quality: Yes
Propagating ccdB Vectors: Not for ccdB vector propagation
F' Episome (to make ssDNA): Lacks F' Episome
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA): No
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Shipping Condition: Dry Ice

Contents & storage

Contains:
• ElectroMAX™ DH5α-E™ Competent Cells: 5 vials, 100 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 2 bottles, 6 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

Documents

Manuals & protocols

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