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Gibco™
DMEM/F-12, HEPES
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Description
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.This DMEM/F-12 is modified as follows:
| With |
| • L-glutamine |
| • HEPES |
| • Phenol Red |
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM/F-12 product made in our Scotland facility (31330-038). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
For In Vitro Diagnostic Use.
Specifications
| Glutamine: | L-Glutamine |
|---|---|
| Phenol Red Indicator: | Phenol Red |
| Form: | Liquid |
| HEPES Buffer: | HEPES |
| Sodium Pyruvate Additive: | Sodium Pyruvate |
| Serum Supplementation: | Standard Serum Supplementation |
| Glucose: | High Glucose |
| Product Line: | Gibco® |
| Product Size: | 10 x 500 mL |
| Volume (Metric): | 500 ml |
| Green Features: | Sustainable packaging |
Contents & storage
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
