This product is manufactured in our Grand Island, US facility and is intended for sale in North America, Latin America, and the Asia/Pacific region—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Paisley, Scotland, is recommended for our customers in Europe, Africa, and the Middle East. Click to view.
      Gibco™

      DMEM/F-12, HEPES This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

      DMEM/F-12, HEPES

      Catalog number:  11330057

       Related applications: Mammalian Cell Culture

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      Description

      DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

      This DMEM/F-12 is modified as follows:
      With
      • L-glutamine
      • HEPES
      • Phenol Red

      The complete formulation is available.

      Using DMEM/F-12
      DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

      cGMP manufacturing and quality system
      DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM/F-12 product made in our Scotland facility (31330-038). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
      For In Vitro Diagnostic Use.

      Specifications

      Glutamine: L-Glutamine
      Phenol Red Indicator: Phenol Red
      Form: Liquid
      HEPES Buffer: HEPES
      Sodium Pyruvate Additive: Sodium Pyruvate
      Serum Supplementation: Standard Serum Supplementation
      Glucose: High Glucose
      Product Line: Gibco®
      Product Size: 10 x 500 mL
      Volume (Metric): 500 ml
      Green Features: Sustainable packaging

      Contents & storage

      Storage conditions: 2°C to 8°C (protect from light)
      Shipping conditions: Ambient
      Shelf life: 12 months from date of manufacture

      Media formulations

      11330 - DMEM/F-12, HEPES

      Documents

      Manuals & protocols

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