Invitrogen™

Dynabeads™ CD8 Positive Isolation Kit

Catalog number: 11333D
Invitrogen™

Dynabeads™ CD8 Positive Isolation Kit

Catalog number: 11333D
Catalog Number
11333D
Unit Size
5 mL
Price (USD)
Price: 1,584.00
Online price: 1,251.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11333D5 mL
Price: 1,584.00
Online price: 1,251.65
Your price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
The Dynabeads™ CD8 Positive Isolation Kit supplies superparamagnetic beads that enable gentle isolation of pure CD8+ T cells from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests. The cells are released from the beads using the DETACHaBEAD™ reagent to obtain bead- and antibody-free cells. Advantages of the Dynabeads™ CD8 Positive Isolation Kit:

• Gentle isolation of CD8+ T cells from any sample—no columns required
• Bead- and antibody-free positive isolation of CD8+ cells for any downstream assay

About Dynabeads™ CD8 Positive Isolation Kit
The Dynabeads™ CD8 Positive Isolation Kit contains uniform, superparamagnetic beads (4.5 μm diameter) covalently coated with a monoclonal antibody specific for the CD8 membrane antigen predominantly expressed on the cytotoxic subset of human T cells. The kit also includes a DETACHaBEAD™ reagent that releases the Dynabeads™ from the purified CD8+ cells. This gently magnetic isolation process gives highly viable bead- and antibody-free cells to be used in any downstream assay.

Magnetic bead–based separation offers easy handling
Dynabeads™ are mixed with the sample in a tube where they bind to the target cells during a short incubation, after which the bead-bound cells are separated from unbound cells using a magnet. The positively isolated cells are then washed, and DETACHaBEAD™ reagent is added to gently release the cells from the beads. This magnetic bead-based positive isolation method gives both bead- and antibody-free cells. The isolated CD8+ cells are phenotypically unaltered and suitable for any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity and viability of the isolated CD8+ cells. For depletion or isolation of untouched CD8+ cells, select from our range of Human CD8+ T Cell Isolation Products.

Learn more about Dynabeads™ products
• Find Dynabeads™ products for a whole range of applications.
• Find magnets for Dynabeads™ separations.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
T Cells (CD8)
Product Type
Positive Cell Isolation Kit
Isolation Technology
Positive Isolation
No. of Cells
Processes ∼2 x 109 cells total
Output Viability
>95%
Product Line
DETACHaBEAD™, DYNAL™, Dynabeads™
Purity or Quality Grade
Research Grade
Sample Type
PBMC, Blood
Shipping Condition
Room Temperature
Starting Material Cell No.
1 x 107 PBMCs per isolation
Target Species
Human
Diameter (Metric)
4.5 μm

Contents & Storage

This Product Contains: 5 mL Dynabeads™ CD8 coated with anti-CD8 monoclonal antibody and 2 mL DETACHaBEAD™ CD8.

Storage Conditions: 2°C to 8°C. Do not freeze.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text