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Invitrogen™

Dynabeads™ Untouched™ Human T Cells Kit

Catalog number: 11344D
Have Questions?
Invitrogen™

Dynabeads™ Untouched™ Human T Cells Kit

Catalog number: 11344D
Have Questions?
Catalog Number
11344D
Unit Size
1 kit
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11344D1 kit
-
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Use this kit to isolate pure and viable untouched T-cells from PBMC by negative isolation. The kit depletes B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free T-cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched T cells
• Easy to use and to scale up
• No columns required

Methodology:
An antibody mix towards the non-T cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human T-cells can be directly analyzed in a flow cytometer and used in any application.

Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended

Starting Sample:
PBMC
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
T Cells (Whole Population)
Product Type
Cell Isolation Kit
Final Product Type
Cells
Isolation Technology
Negative Isolation
No. of Cells
Processes ∼1 x 109 cells total
No. of Tests
100 Isolations
Output Viability
>95%
Product Line
DYNAL™, Dynabeads™, Untouched™
Sample Type
PBMC
Shipping Condition
Room Temperature
Starting Material Cell No.
1 x 107 PBMCs per isolation
Target Species
Human

Contents & Storage

The Kit Contains: 10 mL Depletion Dynabeads™ and 2 mL Antibody Mix with monoclonal antibodies towards human CD14, CD16 (a and b), CD19, CD36, CD56, CD123 and CD235a (Glycophorin A).

Store at 2°C to 8°C.

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