Dynabeads® Untouched™ Human CD4 T Cells Kit

Catalog number: 11346D

Invitrogen™  Related applications: Cell Isolation & Expansion

Error loading your content!

  Catalog number
Select a plan
Unit size
List price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Use this kit to isolate pure and viable untouched CD4+ T-cells from PBMC by negative isolation. The kit depletes CD8+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human CD4+ T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free CD4+ T-cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched CD4+ T cells
• Easy to use and to scale up
• No columns required

Methodology: An antibody mix towards the non-CD4 cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD4+ T-cells can be directly analyzed in a flow cytometer and used in any application.

Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended

Starting Sample:
PBMC
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Capacity: Processes ~1x10^9 cells total
Cell Type: T Cells (CD4)
Clonality: Monoclonal
Product Size: 10 mL
Final Product: Cells
Target Species: Human
Isolation Method: Negative Isolation
Output Viability: >98%, >95%
Number of Reactions: 100 Isolations
Sample Type (Specific): PBMC
Starting Material (Cell #): 1x10^7 PBMCs per isolation
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Shipping Condition: Room Temperature

Contents & storage

The Kit Contains: 10 ml Depletion Dynabeads® and 2 ml Antibody Mix with monoclonal antibodies towards human CD8, CD14, CD16 (a and b), CD19, CD36, CD56, CD123 and CD235a (Glycophorin A).
Store at 2-8°C

Documents

Manuals & protocols

Recommended products