Use this kit to isolate pure and viable untouched CD4+ T-cells from PBMC by negative isolation. The kit depletes CD8+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human CD4+ T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free CD4+ T-cells are truly untouched, and in perfect shape for any functional assay and application.
• High purity, recovery and viability of human untouched CD4+ T cells • Easy to use and to scale up • No columns required
Methodology: An antibody mix towards the non-CD4 cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD4+ T-cells can be directly analyzed in a flow cytometer and used in any application.
Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended
Starting Sample: PBMC
For Research Use Only. Not for use in diagnostic procedures.
T Cells (CD4)
Final Product Type
Not High-throughput Compatible (Manual)
No. of Cells
Processes ∼1x10^9 cells total
No. of Tests
DYNAL™, Dynabeads™, Untouched™
Purity or Quality Grade
Starting Material Cell No.
1x10^7 PBMCs per isolation
Contents & Storage
The Kit Contains: 10 ml Depletion Dynabeads™ and 2 ml Antibody Mix with monoclonal antibodies towards human CD8, CD14, CD16 (a and b), CD19, CD36, CD56, CD123 and CD235a (Glycophorin A). Store at 2-8°C