Dynabeads® Untouched™ Human CD8 T Cells Kit

Catalog number: 11348D

Invitrogen™  Related applications: Cell Isolation & Expansion

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Description

The Dynabeads® Untouched™ Human CD8 T Cells Kit is used to isolate pure and viable untouched CD8+ T cells from PBMC by negative isolation. The kit depletes CD4+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes, and erythrocytes. The negatively isolated human CD8+ T-cells are left in the sample and have not been in contact with the Dynabeads®. The bead- and antibody-free CD8+ T cells are truly untouched, and in perfect shape for any functional assay or application.

Advantages of the Dynabeads® Untouched™ Human CD8 T Cells Kit:

• High purity, recovery, and viability of human untouched CD8+ T cells
• Easy to use and to scale up
• No columns required

Magnetic bead-based Separation offers easy handling
An antibody mix towards the non-CD8 cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD8+ T cells can be directly analyzed in a flow cytometer and used in any application. For depletion or positive isolation of CD8+ cells, select from our range of Human CD8+ T Cell Isolation Products.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Capacity: Processes ~1x10^9 cells total
Cell Type: T Cells (CD8)
Clonality: Monoclonal
Product Size: 1 kit
Final Product: Cells
Target Species: Human
Isolation Method: Negative Isolation
Output Viability: >98%, >95%
Number of Reactions: 100 Isolations
Sample Type (Specific): PBMC
Starting Material (Cell #): 1x10^7 PBMCs per isolation
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Shipping Condition: Room Temperature

Contents & storage

The Kit Contains: 10 ml Depletion Dynabeads® and 2 ml Antibody Mix with monoclonal antibodies towards human CD4, CD14, CD16a, CD16b, CD19, CD36, CD56, CD123 and CD235a (Glycophorin A).

Storage Conditions: 2°C to 8°C. Do not freeze.

Documents

Manuals & protocols

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