Use this kit to isolate pure and viable untouched NK cells from PBMC by negative isolation. The kit depletes T cells, B cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human NK cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free NK cells are truly untouched, and in perfect shape for any functional assay and application.
• High purity, recovery and viability of human untouched NK cells • Easy to use and to scale up • No columns required
Methodology: An antibody mix towards the non-NK cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human NK cells can be directly analyzed in a flow cytometer and used in any application.
Starting Sample: PBMC
For Research Use Only. Not for use in diagnostic procedures.
Cell Isolation Kit
Final Product Type
No. of Cells
No. of Tests
DYNAL™, Dynabeads™, Untouched™
Purity or Quality Grade
Starting Material Cell No.
5 x 107 cells/test
Contents & Storage
The Kit Contains: 10 mL Depletion MyOne™ SA Dynabeads™ and 2 mL Antibody Mix with biotinylated monoclonal antibodies towards human CD3, CD14, CD36, HLA Class II, CD123 and CD235a (Glycophorin A).