Invitrogen™

Dynabeads™ Untouched™ Human NK Cells Kit

Catalog number: 11349D
Invitrogen™

Dynabeads™ Untouched™ Human NK Cells Kit

Catalog number: 11349D
Catalog Number
11349D
Unit Size
1 kit
Price (USD)
Price: 912.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11349D1 kit
Price: 912.00
Your Price:
-
Product Overview
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Additional Information
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Use this kit to isolate pure and viable untouched NK cells from PBMC by negative isolation. The kit depletes T cells, B cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human NK cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free NK cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched NK cells
• Easy to use and to scale up
• No columns required

Methodology:
An antibody mix towards the non-NK cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human NK cells can be directly analyzed in a flow cytometer and used in any application.

Starting Sample:
PBMC
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
NK Cells
Product Type
Cell Kit
Final Product Type
Cells
Format
Bead
Isolation Technology
Negative Isolation
No. of Cells
Processes ∼1x10^9 cells⁄kit
No. of Tests
20
Output Viability
>99%
Product Line
DYNAL™, Dynabeads™, Untouched™
Purity or Quality Grade
Research Grade
Sample Type
MNC
Shipping Condition
Room Temperature
Starting Material Cell No.
∼5x10^7 cells/test
Target Species
Human

Contents & Storage

The Kit Contains: 10 ml Depletion MyOne™ SA Dynabeads™ and 2 ml Antibody Mix with biotinylated monoclonal antibodies towards human CD3, CD14, CD36, HLA Class II, CD123 and CD235a (Glycophorin A).
Store at 2-8°C