Dynabeads® Untouched™ Human B Cells Kit

Catalog number: 11351D

Invitrogen™  Related applications: Cell Isolation & Expansion

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Description

Use this kit to isolate pure and viable untouched B cells from PBMC by negative isolation. The kit depletes T cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched B cells
• Easy to use and to scale up
• No columns required

An antibody mix towards the non-B cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human B cells can be directly analyzed in a flow cytometer and used in any application.

Starting Sample:
PBMC
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Capacity: Processes ~1x10^9 cells⁄kit
Cell Type: B Cells
Final Product: Cells
Form: Bead
Isolation Method: Negative Isolation
Number of Reactions: 20 Reactions
Output Viability: >97%
Product Size: 1 kit
Sample Type (Specific): MNC
Starting Material (Cell #): ~5x10^7 cells/test
Target Species: Human
Shipping Condition: Room Temperature

Contents & storage

The Kit Contains: 10 ml Depletion MyOne™ SA Dynabeads® and 2 ml Antibody Mix with biotinylated monoclonal antibodies towards human CD2, CD14, CD16 (a and b), CD36, CD43 and CD235a (Glycophorin A).
Store at 2-8°C

Documents

Manuals & protocols

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