Dynabeads™ Untouched™ Human CD4 T Cells Kit

      Dynabeads™ Untouched™ Human CD4 T Cells Kit

      Numéro de catalogue:  11352D

      Invitrogen™  Applications liées: Cell Isolation & Expansion

        Contactez-nous pour toute demande d’assistance ›

      Error lors du chargement de votre contenu!

        Numéro de catalogue
      Choisir votre mod�le de prix
      Taille unitaire
      		 Prix ({{currency}}) Disponibilité Qté
      {{product.sku}} {{product.sku}} également connu comme {{product.formattedSku}} 
      		{{product.availability.message}} —
      Ajouts
      Customize this product ›

      Votre stock sur site

      ›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} En stock)
      ›› {{supplyCenter.scName}}(En rupture de stock)
      ›› {{supplyCenter.scName}}
      This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
      Modifier le lieu sélectionné › Commander des produits non stockés
      Haut de la page

      Description

      Use this kit to isolate pure and viable untouched CD4+ T-cells from PBMC by negative isolation. The kit depletes CD8+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human CD4+ T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free CD4+ T-cells are truly untouched, and in perfect shape for any functional assay and application.

      • High purity, recovery and viability of human untouched CD4+ T cells
      • Easy to use and to scale up
      • No columns required

      Methodology: An antibody mix towards the non-CD4 cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD4+ T-cells can be directly analyzed in a flow cytometer and used in any application.

      Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended

      Starting Sample:
      PBMC
      For Research Use Only. Not for use in diagnostic procedures.

      Spécifications

      Capacity: Processes 2x10^8 cells
      Cell Type: T Cells (CD4)
      Final Product: Cells
      Isolation Method: Negative Isolation
      Product Line: DYNAL®, Dynabeads®, Untouched™
      Product Size: 2 mL
      Sample Type (Specific): MNC
      Target Species: Human
      Shipping Condition: Room Temperature

      Contenu et stockage

      The Kit Contains: 2 ml Depletion Dynabeads® and 0.4 ml Antibody Mix with monoclonal antibodies towards human CD8, CD14, CD16 (a and b), CD19, CD36, CD56, CD123 and CD235a (Glycophorin A).
      Store at 2-8°C

      Documents

      Manuels et protocoles

      Produits recommandés

      We use cookies to ensure that we give you the best experience on our website.

      If you continue without changing your settings, we'll assume that you are happy to receive all cookies on Thermo Fisher Scientific websites. However, you can change your cookie settings at any time at the bottom of this page.

      Continue