Dynabeads™ Untouched™ Human CD4 T Cells Kit

Catalog number:  11352D

 Related applications:

Cell Isolation & Expansion

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Use this kit to isolate pure and viable untouched CD4+ T-cells from PBMC by negative isolation. The kit depletes CD8+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human CD4+ T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free CD4+ T-cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched CD4+ T cells
• Easy to use and to scale up
• No columns required

Methodology: An antibody mix towards the non-CD4 cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD4+ T-cells can be directly analyzed in a flow cytometer and used in any application.

Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended

Starting Sample:
For Research Use Only. Not for use in diagnostic procedures.


Capacity: Processes 2x10^8 cells
Cell Type: T Cells (CD4)
Final Product: Cells
Isolation Method: Negative Isolation
Product Line: DYNAL®, Dynabeads®, Untouched™
Product Size: 2 mL
Sample Type (Specific): MNC
Target Species: Human
Shipping Condition: Room Temperature

Contents & storage

The Kit Contains: 2 ml Depletion Dynabeads® and 0.4 ml Antibody Mix with monoclonal antibodies towards human CD8, CD14, CD16 (a and b), CD19, CD36, CD56, CD123 and CD235a (Glycophorin A).
Store at 2-8°C


Manuals & protocols