Dynabeads® FlowComp™ Human CD4 Kit

Catalog number: 11361D

Invitrogen™  Related applications: Cell Isolation & Expansion

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Description

This gentle and easy-to-use kit allows you to isolate flow-compatible human CD4+ T-cells from PBMC or directly from whole blood. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column.
You can expect a purity level of >95% and up to 75% recovery of human CD4+ cells.

Advantages:

• No risk of your results being affected by the isolation method
• Gentle tube-based method - no columns needed
• High purity and recovery
• Bead-free cells - compatible with flow cytometry analysis
• Viable, bead-free CD4+ T-cells for direct use in any cell-based assay.

The isolated cells have the presumed profile of memory and naive CD4+ T cells, and show a high proliferating and functional capacity. This kit avoids contamination of your isolated CD4+ cells with CD14+ monocytes. You will save time and money, and get increased confidence in your results.

Starting sample: MNC or whole blood

Capacity:
The kit will process 2 × 109 mononuclear cells (MNC) or 80 ml whole blood.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Capacity: Processes ~2x10^9 cells total
Cell Type: T Cells (CD4)
Product Size: 3 mL
Target Species: Human
Isolation Method: Positive Isolation
Output Viability: >95%
Sample Type (Specific): Blood, PBMC
Starting Material (Cell #): 5x10^7 PBMCs or 2 ml whole blood per isolation
Shipping Condition: Room Temperature

Contents & storage

The Kit Contains 1 ml Antibody, 3 ml FlowComp™ Dynabeads® and 40 ml FlowComp™ Release Buffer.
Store at: 2-8°C

Documents

Manuals & protocols

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