Dynabeads™ Untouched™ Mouse T Cells Kit

Description

Dynabeads® Untouched™ Mouse T Cells contains both magnetic beads and an Antibody Mix to deplete all leucocytes from mouse spleen or lymph node cells, leaving only the target mouse T cells. The negatively isolated, untouched mouse T-cells are pure, viable and ready to be used in any downstream application.
• Rapid isolation of untouched mouse T-cells – no columns required
• Pure and viable mouse T-cells ready for any downstream application

Excellent Yield, Purity, and Viability of Recovered Cells
The Mouse Depletion Dynabeads® suspension contains uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of cells from any single cell suspension. This reagent is therefore especially well-suited for enriching tissue digests such as mouse spleen and lymph node cells. The Mouse Depletion Dynabeads® are coated with a secondary polyclonal antibody that binds rat IgGs. The Antibody Mix contains a cocktail of rat IgGs that bind mouse B cells, NK cells, monocytes/macrophages, dendritic cells, erythrocytes and granulocytes. The Antibody Mix is first added to the sample in a tube to bind the unwanted non-T cells. The cells are washed to remove excess antibodies, and Mouse Depletion Dynabeads® are then added. After a short incubation, the bead-bound cells are separated within 1 – 2 minutes using a magnet. The purified target mouse T-cells in the supernatant can be transferred to a new tube for use in any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the untouched T cells. The protocol can easily be scaled up according to your sample size.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

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Spécifications

Capacity:	Processes ~1x10^9 cells total
Cell Type:	T Cells (Whole Population)
Isolation Method:	Negative Isolation
Output Viability:	>95%
Product Line:	DYNAL®, Dynabeads®
Product Size:	2 x 10 mL
Reactivity:	Mouse
Sample Type (Specific):	Lymph Node, Spleen
Starting Material (Cell #):	1x10^7 cells per isolation
Target Species:	Mouse
Shipping Condition:	Room Temperature

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