Leibovitz's L-15 Medium
Leibovitz's L-15 Medium
Gibco™

Leibovitz's L-15 Medium

Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue.詳細を見る
製品番号(カタログ番号)数量
1141511410 x 500 mL
11415064500 mL
製品番号(カタログ番号) 11415114
価格(JPY)
36,000
Each
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数量:
10 x 500 mL
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Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO2 equilibration.


This L-15 is modified as follows:
WithWithout
• Galactose• Glucose
• Phenol Red• HEPES
• L-glutamine• Sodium Bicarbonate
• Sodium Pyruvate 


The complete formulation is available.

L-15 contains no proteins, lipids, or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
仕様
細胞株HEP-2 monkey kidney cells
細胞タイプExplants of Embryonic and Adult Human Tissue
濃度1 X
製造品質cGMP-compliant under the ISO 13485 standard
製品ラインGibco
製品タイプLeibovitz's L-15 Medium
数量10 x 500 mL
品質保持期間12 Months From Date of Manufacture
出荷条件Room Temperature
分類Animal Origin
形状Liquid
無菌性Sterile-filtered
Sterilization MethodSterile-filtered
添加剤ありGlutamine, Phenol Red, Sodium Pyruvate, Galactose
添加剤なしNo Glucose, No HEPES, No Sodium Bicarbonate
Unit SizeEach
組成および保存条件
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問(FAQ)

What is the osmolality of Leibovitz's L-15 Medium?

We do provide osmolality information on the certificate of analysis. All lots of Leibovitz's L-15 Medium will meet the osmolality specification of 300-340 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does Leibovitz L-15 medium contain sodium bicarbonate?

No, Leibovitz L15 medium (liquid or powder) does not contain sodium bicarbonate (the formulation for each catalog number is published online). Developed for the support of cell growth in non CO2-equilibrated environments, L-15 formulations were developed without a sodium bicarbonate buffer system. L-15 medium is buffered by phosphates and free-base amino acids (L-arginine primarily, but also L-histidine and L-cysteine). Galactose and sodium pyruvate are used to replace glucose in order to reduce the production of acidic metabolites.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the Leibovitz media you offer?

We offer the following Leibovitz media:

- Leibovitz's L-15 Medium, powder: Cat. Nos. 41300039 (10x 1L) and 41300070 (1x 10L)
- Leibovitz's L-15 Medium, no phenol red: Cat. No 21083027 (1x 500 mL)
- Leibovitz's L-15 Medium: Cat. Nos.11415064 (1x 500 mL) and 11415114 (10x 500 mL) (for sale in N. America, Latin America, and Asia-Pacific regions; Cat. No. 11415049 (for sale in Europe, Africa, and the Middle East
- Leibovitz's L-15 Medium with GlutaMAX Supplement: Cat. No. 31415029 (1x 500 mL)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.