Invitrogen™

Dynabeads™ Untouched™ Mouse CD4 Cells Kit

Catalog number:  11416D

Related applications:

Cell Analysis

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Description

Dynabeads® Untouched™ Mouse CD4 Cells contains both magnetic beads and an Antibody Mix to deplete all leucocytes from mouse spleen or lymph node cells, leaving only the target mouse CD4+ cells. The negatively isolated, untouched mouse CD4+ cells are pure, viable and ready to be used in any downstream application.
• Rapid isolation of untouched mouse CD4+ T-cells – no columns required
• Pure and viable mouse CD4+ T-cells ready for any downstream application

Excellent Yield, Purity, and Viability of Recovered Cells
The Mouse Depletion Dynabeads® suspension contains uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of cells from any single cell suspension. This reagent is therefore especially well-suited for enriching tissue digests such as mouse spleen and lymph node cells. The Mouse Depletion Dynabeads® are coated with a secondary polyclonal antibody that binds rat IgGs. The Antibody Mix contains a cocktail of rat IgGs that binds mouse CD8+ T cells, B cells, NK cells, monocytes/macrophages, dendritic cells, erythrocytes and granulocytes. The Antibody Mix is first added to the sample in a tube to bind the unwanted non-CD4+ cells. The cells are then washed to remove excess antibodies, and Mouse Depletion Dynabeads® are added. After a short incubation, the bead-bound cells are then separated from unbound cells in 1 – 2 minutes using a magnet. The purified target mouse CD4+ cells in the supernatant can be transferred to a new tube for use in any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the untouched CD4+ cells. The protocol can easily be scaled up according to your sample size.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Capacity: Processes 2x10^8 cells
Cell Type: T Cells (CD4)
Final Product Type: Cells
Isolation Technology: Negative Isolation
Product Line: DYNAL®, Dynabeads®
Purity or Quality Grade: Research Grade
Reactivity: Mouse
Shipping Condition: Room Temperature
Target Species: Mouse
Quantity: 4mL
Sample Type: Lymph Node, MNC, Spleen

Contents & storage

The Kit Contains: 4 ml Mouse Depletion Dynabeads® and 0,4 ml Antibody Mix with monoclonal antibodies towards CD8, CD11b, CD16/32, CD45R and Ter-119.
Store at 2-8°C