Invitrogen™

Dynabeads™ Mouse CD43 (Untouched™ B Cells)

Catalog number: 11422D
Invitrogen™

Dynabeads™ Mouse CD43 (Untouched™ B Cells)

Catalog number: 11422D
Catalog Number
11422D
Unit Size
1 kit
Price (USD)
810.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11422D1 kit
810.00
Your Price:
***
Product Overview
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Use this kit to isolate pure and viable untouched B cells from mouse lymphoid organs by negative isolation. The kit depletes T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated mouse B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.

High purity, recovery and viability of mouse untouched B cells

• Easy to use and to scale up
• No columns required

Methodology:
Dynabeads Mouse CD43 (Untouched B Cells) is added directly to the sample and binds the unwanted cells. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched mouse B cells can be directly analyzed in a flow cytometer and used in any application.

Starting Sample:
spleen or lymph nodes
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
B Cells
Final Product Type
Cells
Isolation Technology
Negative Isolation
No. of Tests
40
Product Line
DYNAL™, Dynabeads™, Untouched™
Purity or Quality Grade
Research Grade
Reactivity
Mouse
Shipping Condition
Room Temperature
Starting Material Cell No.
5x10^7 cells⁄test
Target Species
Mouse
Format
Bead
No. of Cells
Processes ∼2x10^9 cells⁄kit, Processes ∼2x10^9 cells total, Processes ∼1x10^9 cells⁄kit
Sample Type
Lymph Node, Tissue Digests, Lymph nodes, Spleen

Contents & Storage

The Kit Contains: 5 ml MyOne™ Dynabeads™- coated with streptavidin and biotinylated rat monoclonal CD43 antibody
against mouse T cells, monocytes⁄macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes.
Store at 2-8°C

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