Use this kit to isolate pure and viable untouched B cells from mouse lymphoid organs by negative isolation. The kit depletes T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated mouse B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.
High purity, recovery and viability of mouse untouched B cells
• Easy to use and to scale up • No columns required
Methodology: Dynabeads Mouse CD43 (Untouched B Cells) is added directly to the sample and binds the unwanted cells. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched mouse B cells can be directly analyzed in a flow cytometer and used in any application.
Starting Sample: spleen or lymph nodes
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell Type
B Cells
Product Type
Antibody Coated Bead
Final Product Type
Cells
Format
Bead
Isolation Technology
Negative Isolation
No. of Cells
Processes ∼2 x 109 cells/kit, Processes ∼2 x 109 cells total, Processes ∼1 x 109 cells/kit
No. of Tests
40
Product Line
DYNAL™, Dynabeads™, Untouched™
Purity or Quality Grade
Research Grade
Reactivity
Mouse
Sample Type
Lymph Node, Tissue Digests, Lymph nodes, Spleen
Shipping Condition
Room Temperature
Starting Material Cell No.
5 x 107 cells/test
Target Species
Mouse
Contents & Storage
The Kit Contains: 5 mL MyOne™ Dynabeads™-coated with streptavidin and biotinylated rat monoclonal CD43 antibodyagainst mouse T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes.