Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool
This MEM is modified as follows:
|With ||Without |
|• Phenol Red ||• L-glutamine |
| ||• HEPES |
The complete formulation
Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2
incubator, or with Hanks' salts for use without CO2
. This product is made with Earle’s salts.Using MEM
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum
(FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2
environment to maintain physiological pH. Concentrated forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol
for details).cGMP Manufacturing and Quality System
Gibco™ MEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ MEM product made in our Scotland facility (21430-020). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.