MaV203 Competent Yeast Cells, Subcloning Scale

Catalog number: 11445012

Invitrogen™  Related applications: Protein Assays and Analysis | Protein Expression

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Description

MaV203 Competent Yeast Cells are designed for use with the ProQuest™ Two-Hybrid System (Figure 1). S. cerevisiae strain MaV203 contains deletions in the endogenous GAL4 and GAL80 genes for use with GAL4-based two-hybrid systems. The strain also has leu2 and trp1 mutations for selection of ProQuest™ bait and prey vectors. MaV203 contains three GAL4 inducible reporter genes, providing four phenotypes, for easier identification of true interactors (Figure 2). The MaV203 competent cells feature:

• Transformation (250 µl of cells) efficiency yields >1 x 106 colonies
• Control DNA pMAB12 to confirm transformation efficiency and the preparation of selection media
• Pre-qualified PEG/Lithium Acetate to maximize number of trans-formants
• Competent cells formatted with carrier DNA already included
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Chemically Competent
Species: S. cerevisiae
Format: Tube(s)
Transformation Efficiency: > 1x10^6
Bacterial or Yeast Strain: MaV203
Blue-White Screening: No
F' Episome (to make ssDNA): Lacks F' Episome
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Improves Plasmid Quality: No
Product Size: 4 x 0.1 mL
Propagating ccdB Vectors: Not for ccdB vector propagation
Shipping Condition: Dry Ice

Contents & storage

Store in ultra-cold freezer (-68 to -85°C).

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Manuals & protocols

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