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Platinum™ GenoType Tsp DNA Polymerase
Platinum GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications.
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| Catalog Number | No. of Reactions |
|---|---|
| 11448024 | 400 Reactions |
| 11448032 also known as 11448-032 | 2000 Reactions |
Catalog number 11448024
Price (USD)/ Each
634.00
-
No. of Reactions:
400 Reactions
Price (USD)/ Each
634.00
Platinum™ GenoType Tsp DNA Polymerase
Catalog number11448024
Price (USD)/ Each
634.00
-
Platinum GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications. The enzyme is pre-complexed with a thermolabile inhibitor containing monoclonal antibodies to Tsp DNA polymerase, providing an automatic hot-start method that improves PCR specificity and allows for room temperature reaction assembly.
Platinum GenoType Tsp DNA Polymerase features
- Minimal non-templated nucleotide addition to PCR products
- Lacks both 5' and 3' exonuclease activity
- Amplifies fragments up to 500 bp
- Room temperature reaction assembly
Application
Amplification of dinucleotide repeat loci in applications in which elimination of non-templated nucleotide addition is desirable.
Unit definition
One unit of Platinum GenoType Tsp DNA Polymerase has been functionally determined to be equivalent to one unit of Taq DNA Polymerase in amplification of dinucleotide repeats using standard Taq DNA polymerase reaction conditions. One Tsp DNA polymerase unit approximates 2.5 activity units. An activity unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min. at 74°C under optimized reaction conditions.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceLow
PolymeraseTsp Genotyping Polymerase
Reaction SpeedStandard
Quantity250 units
Shipping ConditionWet or Dry Ice
For Use With (Application)Hot-start PCR
Fidelity (vs. Taq)1X
Hot StartBuilt-In Hot Start
No. of Reactions400 Reactions
OverhangBlunt
Reaction FormatSeparate Components
Size (Final Product)500 bp or less
Volume50 μL
Unit SizeEach
Contents & Storage
• Platinum GenoType Tsp DNA Polymerase (50 μL at 5 Tsp U/μL)
• 10X PCR Buffer (1.25 mL)
• 50 mM MgCl2 (1 mL)
Store at -20°C. Guaranteed stable for 6 months when properly stored.
• 10X PCR Buffer (1.25 mL)
• 50 mM MgCl2 (1 mL)
Store at -20°C. Guaranteed stable for 6 months when properly stored.