Platinum™ GenoType Tsp DNA Polymerase

      Platinum™ GenoType <i>Tsp</i> DNA Polymerase

      Numéro de catalogue:  11448032

      Invitrogen™  Applications liées: PCR

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      Description

      Platinum® GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications. The enzyme is pre-complexed with a thermolabile inhibitor containing monoclonal antibodies to Tsp DNA polymerase, providing an automatic "hot-start" method that improves PCR specificity and allows for room temperature reaction assembly. Platinum® GenoType Tsp DNA Polymerase:

      • Exhibits minimal non-templated nucleotide addition to PCR products
      • Lacks both 5´ and 3´ exonuclease activity
      • Amplifies fragments up to 500 bp

      Application
      Amplification of dinucleotide repeat loci in applications in which elimination of non-templated nucleotide addition is desirable.

      Unit definition
      One unit of Platinum® GenoType Tsp DNA Polymerase has been functionally determined to be equivalent to one unit of Taq DNA Polymerase in amplification of dinucleotide repeats using standard Taq DNA polymerase reaction conditions. One Tsp DNA polymerase unit approximates 2.5 activity units. An activity unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min at 74°C under optimized reaction conditions.
      For Research Use Only. Not for use in diagnostic procedures.

      Spécifications

      Polymerase: Tsp Genotyping Polymerase
      Hot Start: Built-In Hot Start
      Fidelity (vs. Taq): 1 X
      GC-Rich PCR Performance: Low
      Reaction Format: Separate Components
      Product Overhang: Blunt
      Reaction Speed: Standard
      Final Product Size(s): 500 bp or less
      Exonuclease Activity: None
      Product Line: Platinum®
      Product Size: 2500 units
      Shipping Condition: Approved for shipment on Wet or Dry Ice

      Contenu et stockage

      Contents:
      • 1 tube of 500 µL (5 Tsp U/µL)
      • 10X PCR Buffer (200 mM Tris-HCl pH 8.4, 500 mM KCl)
      • 1 vial of 50 mM MgCl2

      Store at -20°C. Guaranteed stable for 6 months when properly stored.

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