Dynabeads® Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation

Catalog number: 11452D

Gibco™  Related applications: Cell Isolation & Expansion

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Description

Dynabeads® Mouse T-Activator CD3/CD28 are 4.5 µm superparamagnetic beads with anti-CD3 and anti-CD28 antibodies covalently bound to the bead surface. Dynabeads® Mouse T-Activator CD3/CD28 is designed for the activation and expansion of mouse T cells for research purposes.

• Activation of T cells without the need for feeder cells
• Activated cells that retain in vivo-like function
• Maximal reproducibility without contamination by soluble antibodies or mitogens
• Up to 1,000-fold T cell expansion in two weeks

Simple method for T cell activation
Dynabeads® Mouse T-Activator CD3/CD28 offers a simple method for activation and expansion of T cells that does not require feeder cells (antigen-presenting cells) or antigen. The 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T cell activation and expansion. Expansion of the T cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet.

Starting samples
Start with single-cell suspensions from spleen or lymph nodes, mononuclear cells (MNCs), whole blood, or T cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells. Learn more about all of our Dynabeads® cell isolation products.

Downstream applications
The activated T cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: T Cells (Whole Population)
Clonality: Monoclonal
Reactivity: Mouse
Product Size: 2 mL
Final Product: Cells
Target Species: Mouse
Sample Type (General): Cells
Sample Type (Specific): Spleen, Lymph Node, Cell Cultures
Shipping Condition: Room Temperature

Contents & storage

Dynabeads® Mouse T-Activator CD3/CD28 are supplied as a suspension containing 4 × 107 Dynabeads®/ml in phosphate buffered saline (PBS), pH 7.4, with 0.1% bovine serum albumin (HSA). Store at: 2-8°C.

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Manuals & protocols

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