Dynabeads® Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation

Catalog number: 11456D

Gibco™  Related applications: Cell Isolation & Expansion

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Description

Dynabeads® Mouse T-Activator CD3/CD28 is for the activation and expansion of mouse T-cells for research purposes.

With Dynabeads® Mouse T-Activator CD3/CD28 you get:

• Activation of T-cells without the need of feeder cells
• Activated cells that retain in vivo-like function
• Maximal reproducibility without contamination by soluble antibodies or mitogens
• From 100- to 1000-fold T-cell expansion

How Dynabeads® Mouse T-Activator CD3/CD28 works:
Dynabeads® Mouse T-Activator CD3/CD28 offers a simple method for activation and expansion of T-cells that does not require feeder cells (antigen-presenting cells) or antigen. The 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T-cell activation and expansion. Expansion of the T-cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet.

For expansion of antigen specific T-cells from T-cell clones or T-cell lines, we recommend using Dynabeads® Mouse T-Activator CD3/CD28/CD137.

Starting samples
Start with single cell suspensions from spleen or lymph nodes, mononuclear cells (MNCs), whole blood, or T-cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells. Get an overview of the different Dynabeads® available for cell isolation.

Downstream applications
The activated T-cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T-cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Bead
Ligand: Antibody
Cell Type: T Cells (Whole Population)
Clonality: Monoclonal
Reactivity: Mouse
Host Species: Hamster
Product Size: 0.4 mL
Final Product: Activated, expanded T cells
Target Species: Mouse
Isolation Method: Dynabeads Untouched or Dynabeads FlowComp
Number of Reactions: 200 Tests
Surface Functionality: Epoxy
Sample Type (Specific): Spleen, Lymph Node
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Shipping Condition: Room Temperature

Contents & storage

0.4 ml Dynabeads® Mouse T-Activator CD3/CD28 is supplied as a suspension containing 4 × 107 Dynabeads®/ml in phosphate buffered saline (PBS), pH 7.4, with 0.1% bovine serum albumin (BSA). Store at: 2–8°C.

Documents

Manuals & protocols

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