Cell cycle-regulated phosphorylation of the Xenopus polo-like kinase Plx1.
Authors Kelm Olaf; Wind Mathias; Lehmann Wolf D; Nigg Erich A;
JournalJ Biol Chem
PubMed ID11994303
'Polo-like kinases (Plks) control multiple important events during M phase progression, but little is known about their activation during the cell cycle. The activities of both mammalian Plk1 and Xenopus Plx1 peak during M phase, and this activation has been attributed to phosphorylation. However, no phosphorylation sites have previously been ... More
Variola virus immune evasion design: expression of a highly efficient inhibitor of human complement.
Authors Rosengard Ariella M; Liu Yu; Nie Zhiping; Jimenez Robert;
JournalProc Natl Acad Sci U S A
PubMed ID12077314
'Variola virus, the most virulent member of the genus Orthopoxvirus, specifically infects humans and has no other animal reservoir. Variola causes the contagious disease smallpox, which has a 30-40% mortality rate. Conversely, the prototype orthopoxvirus, vaccinia, causes no disease in immunocompetent humans and was used in the global eradication of ... More
Alternate interactions define the binding of peptides to the MHC molecule IA(b).
Authors Liu Xinqi; Dai Shaodong; Crawford Frances; Fruge Rachel; Marrack Philippa; Kappler John;
JournalProc Natl Acad Sci U S A
PubMed ID12084926
'We have solved the crystal structure of the MHCII molecule, IA(b), containing an antigenic variant of the major IA(b)-binding peptide derived from the MHCII IEalpha chain. The four MHC pockets at p1, p4, p6, and p9 that usually bind peptide side chains are largely empty because of alanines in the ... More
Identification and characterization of three members of the human metallocarboxypeptidase gene family.
'Amino acid homology searches of the human genome revealed three members of the metallocarboxypeptidase (metallo-CP) family that had not been described in the literature in addition to the 14 known genes. One of these three, named CPA5, is present in a gene cluster with CPA1, CPA2, and CPA4 on chromosome ... More
Conversion of proepithelin to epithelins: roles of SLPI and elastase in host defense and wound repair.
Increased leukocyte elastase activity in mice lacking secretory leukocyte protease inhibitor (SLPI) leads to impaired wound healing due to enhanced activity of TGFbeta and perhaps additional mechanisms. Proepithelin (PEPI), an epithelial growth factor, can be converted to epithelins (EPIs) in vivo by unknown mechanisms with unknown consequences. We found that ... More
Characterization of RAC3, a novel member of the Rho family.
AuthorsHaataja L, Groffen J, Heisterkamp N
JournalJ Biol Chem
PubMed ID9252344
The small GTP-binding proteins Rac1 and Rac2 are critically important in regulating multiple signal transduction pathways in eukaryotic cells. Here we report the isolation of a novel third Rac family member, Rac3. Rac3 differs from Rac1/2 at its carboxyl-terminal end, a domain associated with subcellular localization and binding to specific ... More
All Six Modules of the Gelatin-binding Domain of Fibronectin Are Required for Full Affinity.
AuthorsKatagiri Y, Brew SA, Ingham KC,
JournalJ Biol Chem
PubMed ID12538576
The gelatin-binding sites of fibronectin are confined to a 42-kDa region having four type I and two type II modules in the following order: I(6)-II(1)-II(2)-I(7)-I(8)-I(9). To determine the relative importance of each module for recognition of gelatin, recombinant green fluorescent fusion proteins were prepared in which individual modules or groups ... More
Human exonuclease I is required for 5' and 3' mismatch repair.
Authors Genschel Jochen; Bazemore Laura R; Modrich Paul;
JournalJ Biol Chem
PubMed ID11809771
We have partially purified a human activity that restores mismatch-dependent, bi-directional excision to a human nuclear extract fraction depleted for one or more mismatch repair excision activities. Human EXOI co-purifies with the excision activity, and the purified activity can be replaced by near homogeneous recombinant hEXOI. Despite the reported 5' ... More
COX-3, a cyclooxygenase-1 variant inhibited by acetaminophen and other analgesic/antipyretic drugs: cloning, structure, and expression.
Authors Chandrasekharan N V; Dai Hu; Roos K Lamar Turepu; Evanson Nathan K; Tomsik Joshua; Elton Terry S; Simmons Daniel L;
JournalProc Natl Acad Sci U S A
PubMed ID12374850
Two cyclooxygenase isozymes, COX-1 and -2, are known to catalyze the rate-limiting step of prostaglandin synthesis and are the targets of nonsteroidal antiinflammatory drugs. Here we describe a third distinct COX isozyme, COX-3, as well as two smaller COX-1-derived proteins (partial COX-1 or PCOX-1 proteins). COX-3 and one of the ... More
High-level expression and purification of secreted forms of herpes simplex virus type 1 glycoprotein gD synthesized by baculovirus-infected insect cells.
Authors Sisk W P; Bradley J D; Leipold R J; Stoltzfus A M; Ponce de Leon M; Hilf M; Peng C; Cohen G H; Eisenberg R J;
JournalJ Virol
PubMed ID8289380
Two forms of herpes simplex virus glycoprotein gD were recombined into Autographa californica nuclear polyhedrosis virus (baculovirus) and expressed in infected Spodoptera frugiperda (Sf9) cells. Each protein was truncated at residue 306 of mature gD. One form, gD-1(306t), contains the coding sequence of Patton strain herpes simplex virus type 1 ... More