Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis and pyroptosis.
AuthorsFisch D, Bando H, Clough B, Hornung V, Yamamoto M, Shenoy AR, Frickel EM
JournalEMBO J
PubMed ID31268602
'The guanylate binding protein (GBP) family of interferon-inducible GTPases promotes antimicrobial immunity and cell death. During bacterial infection, multiple mouse Gbps, human GBP2, and GBP5 support the activation of caspase-1-containing inflammasome complexes or caspase-4 which trigger pyroptosis. Whether GBPs regulate other forms of cell death is not known. The apicomplexan ... More
Id1 Ablation Protects Hematopoietic Stem Cells from Stress-Induced Exhaustion and Aging.
AuthorsSingh SK, Singh S, Gadomski S, Sun L, Pfannenstein A, Magidson V, Chen X, Kozlov S, Tessarollo L, Klarmann KD, Keller JR
JournalCell Stem Cell
PubMed ID30082068
'Defining mechanisms that maintain tissue stem cells during homeostasis, stress, and aging is important for improving tissue regeneration and repair and enhancing cancer therapies. Here, we show that Id1 is induced in hematopoietic stem cells (HSCs) by cytokines that promote HSC proliferation and differentiation, suggesting that it functions in stress ... More
Generation of Mutants of Nuclear-Encoded Plastid Proteins Using CRISPR/Cas9 in the Diatom Phaeodactylum tricornutum.
AuthorsAllorent G, Guglielmino E, Giustini C, Courtois F
JournalMethods Mol Biol
PubMed ID29987734
Genome modifications in microalgae are becoming a widespread and mandatory tool for research in both fundamental and applied biology. Among genome editing methods in these photosynthetic organisms, CRISPR/Cas9 offers a specific, powerful and efficient tool for genome engineering by inducing mutations in targeted regions of the genome. Here we described ... More
Completeness of HIV-1 Envelope Glycan Shield at Transmission Determines Neutralization Breadth.
AuthorsWagh K, Kreider EF, Li Y, Barbian HJ, Learn GH, Giorgi E, Hraber PT, Decker TG, Smith AG, Gondim MV, Gillis L, Wandzilak J, Chuang GY, Rawi R, Cai F, Pellegrino P, Williams I, Overbaugh J, Gao F, Kwong PD, Haynes BF, Shaw GM, Borrow P, Seaman MS, Hahn BH, Korber B
JournalCell Rep
PubMed ID30355496
Densely arranged N-linked glycans shield the HIV-1 envelope (Env) trimer from antibody recognition. Strain-specific breaches in this shield (glycan holes) can be targets of vaccine-induced neutralizing antibodies that lack breadth. To understand the interplay between glycan holes and neutralization breadth in HIV-1 infection, we developed a sequence- and structure-based approach ... More
Cannabidiol directly targets mitochondria and disturbs calcium homeostasis in acute lymphoblastic leukemia.
AuthorsOlivas-Aguirre M, Torres-López L, Valle-Reyes JS, Hernández-Cruz A, Pottosin I, Dobrovinskaya O
JournalCell Death Dis
PubMed ID31611561
Anticancer properties of non-psychoactive cannabinoid cannabidiol (CBD) have been demonstrated on tumors of different histogenesis. Different molecular targets for CBD were proposed, including cannabinoid receptors and some plasma membrane ion channels. Here we have shown that cell lines derived from acute lymphoblastic leukemia of T lineage (T-ALL), but not resting ... More
Accurate and live peroxisome biogenesis evaluation achieved by lentiviral expression of a green fluorescent protein fused to a peroxisome targeting signal 1.
AuthorsDemaret T, Courtoy GE, Ravau J, Van Der Smissen P, Najimi M, Sokal EM
JournalHistochem Cell Biol
PubMed ID32124009
Peroxisomes are ubiquitous organelles formed by peroxisome biogenesis (PB). During PB, peroxisomal matrix proteins harboring a peroxisome targeting signal (PTS) are imported inside peroxisomes by peroxins, encoded by PEX genes. Genetic alterations in PEX genes lead to a spectrum of incurable diseases called Zellweger spectrum disorders (ZSD). In vitro drug ... More
CRISPR-Cas13 Inhibitors Block RNA Editing in Bacteria and Mammalian Cells.
AuthorsLin P, Qin S, Pu Q, Wang Z, Wu Q, Gao P, Schettler J, Guo K, Li R, Li G, Huang C, Wei Y, Gao GF, Jiang J, Wu M
JournalMol Cell
PubMed ID32348779
Cas13 has demonstrated unique and broad utility in RNA editing, nucleic acid detection, and disease diagnosis; however, a constantly active Cas enzyme may induce unwanted effects. Bacteriophage- or prophage-region-encoded anti-CRISPR (acr) gene molecules provide the potential to control targeting specificity and potency to allow for optimal RNA editing and nucleic ... More