Invitrogen™

ElectroMAX™ Stbl4™ Competent Cells

Catalog number: 11635018
Invitrogen™

ElectroMAX™ Stbl4™ Competent Cells

Catalog number: 11635018
Catalog Number
11635018
also known as 11635-018
Unit Size
5 x 100 µL
Price (USD)
347.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11635018
also known as 11635-018
5 x 100 µL
347.00
Your Price:
***
Product Overview
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Additional Information
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Recommendations
ElectroMAX™ Stbl4™ Competent Cells are specifically designed for cloning unstable inserts. As electrocompetent cells, they have one of the highest transformation efficiencies available (>5×109 cfu/µg), making them ideal for generating cDNA and genomic libraries and for cloning unstable inserts. These electrocompetent E. coli:

• Are ideal for cloning unstable DNA—stabilizes direct repeat and retroviral sequences
• Permit efficient cloning of methylated genomic DNA
• Support blue/white screening
• Are designed to deliver high-yield plasmid preparations for downstream applications
• Provide transformation efficiencies of >5×109 cfu/µg

Propagating unstable and large DNA with high transformation-efficiency cells
Many competent cell strains have the recA1 genotype, which reduces recombination. However there are some instances when the DNA that you are trying to clone is still unstable in such cells, perhaps due to the presence of inverted or direct repeats, or GC-rich tracts. While such sequences are relatively common in eukaryotic genomes, they are rare in E. coli. Consequently, rearrangements may occur when these sequences are introduced into standard E. coli strains.

Using ElectroMAX™ Stbl4™ Cells
ElectroMAX™ Stbl4™ electrocompetent E. coli cells are a derivative of Stbl2™ cells and are ideal for the cloning of unstable inserts such as retroviral sequences, direct repeats, and tandem array genes. Furthermore, ElectroMAX™ Stbl4™ cells are useful for the generation of cDNA libraries using plasmid-derived vectors and are able to take up and maintain large plasmids (e.g., 50 kb cosmids and 100–200 kb P1 clones). The Stbl4™ cells also contain an F' episome, allowing them to serve as a host for single-stranded DNA such as M13mp cloning vectors. The lacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors, and can therefore be used for blue/white screening of colonies on agar plates containing Xgal or Bluo-gal and IPTG. The mcrA mutation and the mcrBC-hsdRMS-mrr deletion allow cloning of genomic sequences which are methylated. Finally, the endA1 mutation greatly increases plasmid yield and quality.

Genotype: mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96 gal-thi-1 supE44 λ-relA1 Δ(lac-proAB)/F' proAB+lacIqZΔM15 Tn10 (TetR)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
HEK 293 Cell
Contains F' Episome
Contains F' Episome
Efficiency
High Efficiency (>10^9 cfu/µg)
Cell Type
Electrocompetent
Improves Plasmid Quality
Yes
Cloning Methylated DNA
Yes
Transformation Efficiency Level
High Efficiency (> 10^9 cfu⁄µg)
Cloning Unstable DNA
Suitable for cloning unstable DNA
Bacterial or Yeast Strain
Stbl4™
Blue/White Screening
Yes
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Plasmid
May be used for plasmids > 100 kb
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
Yes
Shipping Condition
Dry Ice
T1 Phage - Resistant (tonA)
No
Species
E. coli
Format
Tube
Product Line
ElectroMAX™, Stbl4™
Quantity
5 x 100 μL

Contents & Storage

Contains:
• ElectroMAX™ Stbl4™ Competent Cells: 5 vials, 100 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 2 bottles, 6 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

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