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AIM2 Recombinant Human Protein, N-GST tag

Catalog number:  11654H09B250

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Description

Absent In Melanoma 2 (AIM2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications

This recombinant protein was expressed from a DNA sequence encoding the human AIM2 (NP_004824.1) (Met 1-Thr 343) fused to the GST tag at the N-terminus.

Activity: Measured by its ability to inhibit the proliferation of MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083). The ED50 for this effect is typically 4-7 µg/ml.

Formulation: Lyophilized in 50 mM Tris, 1M NaCL, 0.5 mM PMSF, 5 mM GSH, pH 8.0, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Expression: Insect
Form: Lyophilized
Gene Aliases: AIM2, RP11-520H16.3, PYHIN4
Gene Symbol: AIM2
Molecular Weight: 65.2 kDa
Product Line: Sino Biological®
Product Size: 5 x 50 µg
Protein Family: Other Proteins
Protein Name: absent in melanoma 2
Protein Subtype: NFκB Pathway Proteins
Protein Tag or Fusion: GST Tag
Purity: ≥ 90% by SDS-PAGE
Research Category: Inflammation, Oncology, Signal Transduction
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols